HSD17B10, RPL8, IFITM2 and IFITM3 as predictive markers of response to chemotherapy in conventional osteosarcomas.

ABSTRACT: The aim of this study was to identify molecular markers able to classify osteosarcoma patients as good or poor responders before therapy. Methods: Gene expression profiling of 20 non-metastatic high-grade osteosarcoma patients was performed using cDNA nylon microarray which contained 8,032 unique sequenced-verified cDNA clones, representing 5,776 distinct genes. Expression of selected genes was validated using RTQ-PCR. For correlated genes, corresponding proteins were explored. Immunohistochemistry (IHC) on sections of tissue microarrays (TMA) were performed on a cohort of 50 patients, out of which 30 were new patients. Furthermore, fluorescent in situ hybridization (FISH) was performed on gene which could not be tested using IHC. Results: We have found that HSD17B10 gene expression was up

ORGANISM(S): Homo sapiens

SUBMITTER: Wilfried Gouraud 

PROVIDER: E-GEOD-11127 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress