Transcription profiling of mouse single cells from primordial germ cell lineage (E6.25-E8.25, wild type and Blimp1KO)

ABSTRACT: Specification of germ cell fate is fundamental in development. With a highly representative single-cell microarray and rigorous quantitative-PCR analysis, we defined the genome-wide transcription dynamics that create primordial germ cells (PGCs) from the epiblast, a process that exclusively segregates them from their somatic neighbors. We also analyzed the effect of the loss of Blimp1, a key transcriptional regulator, on these dynamics. Our analysis revealed that PGC specification involves complex, yet highly ordered regulation of a large number of genes, proceeding under the strong influence of mesoderm induction with active repression of specific programs such as epithelial-mesenchymal transition, Hox gene activation, cell-cycle progression and DNA methyltransferase machinery. Remark

ORGANISM(S): Mus musculus

SUBMITTER: Kazuki Kurimoto 

PROVIDER: E-GEOD-11128 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress