Transcription profiling of rat folliculogenesis reveals glial derived neurotrophic factor promotes ovarian primordial follicle development

ABSTRACT: Female fertility is determined in part by the size and development of the primordial follicle pool. The current study investigates the role of glial cell-line derived neurotrophic factor (GDNF) in the regulation of primordial follicle development in the ovary. Ovaries from four-day old female rat pups were maintained in organ culture for ten days in the absence (control) or presence of GDNF or kit ligand/stem cell factor (KL). Ovaries treated with GDNF contained a significant increase in developing follicles, similar to that observed with KL treatment previously shown to promote follicle development. The actions of GDNF on the ovarian transcriptome were investigated with a microarray analysis. Immunohistochemical studies demonstrated that GDNF is localized to oocyte cytoplasm in follicles

ORGANISM(S): Rattus norvegicus

SUBMITTER: Michael Skinner 

PROVIDER: E-GEOD-11133 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress