Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Hydrogenomics of the extremely thermophilic bacterium Caldicellulosiruptor saccharolyticus

ABSTRACT: Caldicellulosiruptor saccharolyticus is an extremely thermophilic, Gram-positive anaerobe, which ferments cellulose-, hemicellulose- and pectin-containing biomass to acetate, CO2 and hydrogen. Its broad substrate range, high hydrogen-producing capacity, and ability to co-utilize glucose and xylose, make this bacterium an attractive candidate for microbial bioenergy production. Glycolytic pathways and an ABC-type sugar transporter were significantly up-regulated during growth on glucose and xylose, indicating that C. saccharolyticus co-ferments these sugars unimpeded by glucose-based catabolite repression. The capacity to simultaneously process and utilize a range of carbohydrates associated with biomass feedstocks represents a highly desirable feature of a lignocellulose-utilizing, biofuel-producing bacterium. Keywords: substrate response C. saccharolyticus was subcultured (overnight) 3 times on the substrate of interest in modified DSMZ 640 medium before inoculating a pH-controlled (pH = 7) 1-liter fermentor containing 4 gram substrate per liter. Cells were grown at 70 °C until mid-logarithmic phase (~OD660 = 0.3-0.4) and harvested by centrifugation and rapid cooling to 4 °C and stored at -80 °C. To elucidate the central carbon metabolic pathways and their regulation, transcriptome analysis was performed after growth on glucose, xylose and a 1:1 mixture of both substrates. L-Rhamnose, which was likely to follow another pathway, was used as a reference substrate.

ORGANISM(S): Caldicellulosiruptor saccharolyticus

SUBMITTER: Amy VanFossen

PROVIDER: E-GEOD-11153 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Hydrogenomics of the extremely thermophilic bacterium Caldicellulosiruptor saccharolyticus.

van de Werken Harmen J G HJ Verhaart Marcel R A MR VanFossen Amy L AL Willquist Karin K Lewis Derrick L DL Nichols Jason D JD Goorissen Heleen P HP Mongodin Emmanuel F EF Nelson Karen E KE van Niel Ed W J EW Stams Alfons J M AJ Ward Donald E DE de Vos Willem M WM van der Oost John J Kelly Robert M RM Kengen Servé W M SW

Applied and environmental microbiology 20080905 21

Caldicellulosiruptor saccharolyticus is an extremely thermophilic, gram-positive anaerobe which ferments cellulose-, hemicellulose- and pectin-containing biomass to acetate, CO(2), and hydrogen. Its broad substrate range, high hydrogen-producing capacity, and ability to coutilize glucose and xylose make this bacterium an attractive candidate for microbial bioenergy production. Here, the complete genome sequence of C. saccharolyticus, consisting of a 2,970,275-bp circular chromosome encoding 2,67 ...[more]

PMID: 18776029

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Dataset Information

Hydrogenomics of the extremely thermophilic bacterium Caldicellulosiruptor saccharolyticus

Publications

Hydrogenomics of the extremely thermophilic bacterium Caldicellulosiruptor saccharolyticus.

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