Transcription profiling of mouse CD11b+ cells after infection of lethal and sublethal wild type Yersinia enterolitica vs controls reveals IFN-g counteracts YopH mediated immune evasion

ABSTRACT: Background:; Yersinia outer protein (Yop) H is a secreted virulence factor of Yersinia enterocolitica which inhibits phagocytosis of Y. enterocolitica and promotes virulence of Y. enterocolitica (Ye) in mice. The aim of this study was to address whether and how YopH affects the innate immune response against Ye in mice. Results:; For this purpose mice were infected with wild type Ye (pYV+) or a YopH-deficient Ye mutant strain (DyopH). CD11b+ cells were isolated from infected spleen and subjected to gene expression analysis using microarrays. Despite attenuation of DyopH in vivo, by variation of infection doses we were able to achieve conditions that allow comparison of gene expression in pYV+ and DyopH infections at either comparable infection courses or splenic bacterial burden. Gene expr

ORGANISM(S): Mus musculus

SUBMITTER: Erwin Bohn 

PROVIDER: E-GEOD-11189 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress