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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of pig muscle longissimus dorsi sample of f2 animals, T-test paired and SAM

ABSTRACT: To identify biological processes as well as molecular markers for drip loss, the transcriptomes of logissimus dorsi from 6 sib pair of F2 animals Experiment Overall Design: 12 F2 animals of a resource population based on the breeds Duroc and Pietrain known to differ in meat quality and quantity traits were hybridized to Affymetrix Porcine Genome Arrays

ORGANISM(S): Sus scrofa

SUBMITTER: Klaus Wimmers

PROVIDER: E-GEOD-11193 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression data from Muscle Longissimus dorsi sample of F2 animals, T-test paired and SAM

Project description:To identify biological processes as well as molecular markers for drip loss, the transcriptomes of logissimus dorsi from 6 sib pair of F2 animals Keywords: T-test paired and SAM

2009-01-01 | GSE11193 | GEO

Transcription profiling of pig muscle longissimus dorsi sample from F2 animals

Project description:In this study, we used correlation analysis of the expression profiles and drip loss to produce a list of functional candidate genes under the assumption that genes with strong correlation between their expression values and drip belong to pathways or networks relevant for the control of the trait. Expression QTL analyses for transcripts showing trait dependent expression were done in order to identify genes which show trait associated expression, map to pQTL regions, and exhibit cis regulation. Experiment Overall Design: 74 F2 animals of a resource population based on the breeds Duroc and Pietrain known to differ in meat quality and quantity traits were hybridized to Affymetrix Porcine Genome Arrays.

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Consequences of a low protein diet in the liver and longissimus dorsi transcriptome of Duroc x Iberian crossbred pigs

Project description:Low protein diets supplied during the growing period of pigs can diminish their growth rate and increase the intramuscular fat (IMF) content which affects the sensorial and technological characteristics of the traits. In the present study, the effects of a low protein diet supplied during the growing diet of Duroc x Iberian crossbred pigs on several phenotypic traits and liver and longissimus dorsi transcriptome, were analysed 20 days after the differential treatment was started (EARLY) and at the end of it (FINAL). A total of 20 crossbred pigs were assigned to two different dietary treatments during the growing period: a control diet (C) and a low protein diet (LP) with the same energy and lower levels of raw protein (11%) and lysine (0.60%). The transcriptomes of liver and longissimus dorsi were quantified through RNAseq. A total of 134 differentially expressed annotated genes and new isoforms (DEGs) between C and LP diets in liver of EARLY animals; 480 DEGs in liver of LATE animals, and 128 DEGs and 68 DEGs in longissimus dorsi of EARLY and LATE animals were detected. The functional analyses revealed that low protein diet diminishes the expression in liver of genes codifying for proteins involved in immune system both in EARLY and LATE animals, affects the expression of genes involved in cholesterol homeostasis in liver and in the energy process and growth in longissimus dorsi. Pigs fed with LP diet had not higher IMF content than C ones, although some lipogenesis genes such as FASN, SCD or SREBF1 were higher expressed on their liver. A low protein diet supplied during growing period affects multiple biological process that could compromise the immune and energy state of the Duroc x Iberian crossbred pigs. These results point out that we should be very cautious before implementing this type of regime in Duroc x Iberian pigs.

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Comparative analysis of Musculus longissimus dorsi expression of Holstein x Charolais F2 cattle (SEGFAM) with high and low intramuscular fat (IMF) content

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Genome-wide linkage analysis of global gene expression of loin muscle tissue identifies putatively cis-acting candidate genes in pigs

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