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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Tandem duplication producing a novel oncogenic BRAF fusion gene defines the majority of pilocytic astrocytomas

ABSTRACT: Pilocytic astrocytomas (PAs), WHO Grade I, are one of the most frequently occurring childhood brain tumors. We have used microarray comparative genomic hybridization (aCGH) to study copy number changes on chromosome 7 in a series of PAs (n=44). Keywords: Comparative Genomic Hybridization Single hybridization per case. 44 pilocytic astrocytomas WHO grade I were analyzed. Target (tumor) labelled with Cy5 and reference with Cy3. Mixture of 20 normal male or female genomic DNA was used in sex-mismatched hybridization.

ORGANISM(S): Homo sapiens

SUBMITTER: David Jones

PROVIDER: E-GEOD-11265 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Tandem duplication producing a novel oncogenic BRAF fusion gene defines the majority of pilocytic astrocytomas.

Jones David T W DT Kocialkowski Sylvia S Liu Lu L Pearson Danita M DM Bäcklund L Magnus LM Ichimura Koichi K Collins V Peter VP

Cancer research 20081101 21

Brain tumors are the most common solid tumors of childhood, and pilocytic astrocytomas (PA) are the most common central nervous system tumor in 5 to 19 year olds. Little is known about the genetic alterations underlying their development. Here, we describe a tandem duplication of approximately 2 Mb at 7q34 occurring in 66% of PAs. This rearrangement, which was not observed in a series of 244 higher-grade astrocytomas, results in an in-frame fusion gene incorporating the kinase domain of the BRAF ...[more]

PMID: 18974108

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