Genome-Wide Microarray Analysis Reveals Massive Changes in Gene Expression During Conjugation in Tetrahymena thermophila

ABSTRACT: A genome wide microarray platform containing the predicted coding sequences (putative genes) for the ciliated protozoan Tetrahymena thermophila is described, validated and used to study gene expression in growing, starved and conjugating cells. Of the ~27,400 predicted open reading frames in Tetrahymena, transcripts homologous to 5876 are not detectable in one or more of these life cycle stages, indicating that this organism does indeed contain a large number of functional genes. Transcripts from over 5000 predicted genes are expressed at levels 5X background and 95 genes are expressed at 250X background in all stages. Transcripts homologous to 94 predicted genes are specifically expressed and 155 more are highly up-regulated in growing cells, and 90 are specifically expressed and 616 are

ORGANISM(S): Tetrahymena thermophila

SUBMITTER: Martin Gorovsky 

PROVIDER: E-GEOD-11300 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress