Transcription profiling by array time series of human MCF7 breast cancer cells treated with estradiol

ABSTRACT: Using a chromatin immunoprecipitation-paired end diTag cloning and sequencing strategy, we mapped estrogen receptor alpha (ERalpha) binding sites in MCF-7 breast cancer cells. We identified 1,234 high confidence binding clusters of which 94% are projected to be bona fide ERalpha binding regions. Only 5% of the mapped estrogen receptor binding sites are located within 5 kb upstream of the transcriptional start sites of adjacent genes, regions containing the proximal promoters, whereas vast majority of the sites are mapped to intronic or distal locations (>5 kb from 5' and 3' ends of adjacent transcript), suggesting transcriptional regulatory mechanisms over significant physical distances. Of all the identified sites, 71% harbored putative full estrogen response elements (EREs), 25% bore ERE

ORGANISM(S): Homo sapiens

SUBMITTER: Lance David Miller 

PROVIDER: E-GEOD-11352 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress