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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse early and aduly developmental stages

ABSTRACT: DNA repair genes have been shown to be expressed in the early stages of mammalian development probably to reduce possible replication errors and genotoixc damages. Several birth defects and some cancers are due to inappropriate or defective DNA repair machinery indicating that a right activity of DNA repair genes in the early stages of fetal development are essential for an appropriate DNA function. Neuroblastoma (NB), an embryonal tumor deriving from neural crest cells (NCCs) is diagnosed in about 30% of patients within the first year of life. Moreover, several reports show that NB can be detected in foetus and in neonatal period. To assess gene expression profiling of DNA repair genes during early stage of development, we performed a genome-wide analysis of Neural Crest cells (NCCs) generating a gene expression database that can help to better understand the gene(s) involved in both genetic and cancer diseases. We found 11 genes involved in DNA repair activity during mouse embryo development overexpressed in early stages. Six out 11 were never been described in mouse embryology. Experiment Overall Design: Mouse embryos were collected at successive stages of early and adult development stages for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain homogeneous populations of NCCs by Laser Capture Microdissection (LCM) from embryos and adult in order to analyze DNA repair genes during mouse development. To that end, we collected cells at three time-points: at embryonal stage of 8.5 dpc: E8.5 (neural tube closure); at embryonal stage of 13.5dpc: E13.5 (dorsal root ganglion); at adult stage of 3 months postnatal: P90 (adrenal medulla).

ORGANISM(S): Mus musculus

SUBMITTER: Domenico Albino

PROVIDER: E-GEOD-11356 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data from early and adult stages of mouse development

Project description:DNA repair genes have been shown to be expressed in the early stages of mammalian development probably to reduce possible replication errors and genotoixc damages. Several birth defects and some cancers are due to inappropriate or defective DNA repair machinery indicating that a right activity of DNA repair genes in the early stages of fetal development are essential for an appropriate DNA function. Neuroblastoma (NB), an embryonal tumor deriving from neural crest cells (NCCs) is diagnosed in about 30% of patients within the first year of life. Moreover, several reports show that NB can be detected in foetus and in neonatal period. To assess gene expression profiling of DNA repair genes during early stage of development, we performed a genome-wide analysis of Neural Crest cells (NCCs) generating a gene expression database that can help to better understand the gene(s) involved in both genetic and cancer diseases. We found 11 genes involved in DNA repair activity during mouse embryo development overexpressed in early stages. Six out 11 were never been described in mouse embryology. Keywords: time course

2008-05-06 | GSE11356 | GEO

CHAF1A Blocks Neuronal Differentiation and Promotes Neuroblastoma Oncogenesis via Metabolic Reprogramming

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MYCN induces neuroblastoma in primary neural crest cells

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The BAF chromatin remodeling complex regulates gene expression programs essential for mammalian neural crest development

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