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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling by array of human aortic vascular smooth muscle cells treated with IL-17 or untreated

ABSTRACT: Investigate the effect of recombinant human IL-17A on vascular smooth muscle cells cultured from human aortas. Experiment Overall Design: SMCs from the aortas of three different donors were cultured in M199 media supplemented with 20% FCS and used at passage 3. The cells were either not treated or treated with IL-17 at 100 ng/ml for 6 hr. The six samples were labeled as Untreated or IL-17-treated from three independent experiments labeled A, B, and C.

ORGANISM(S): Homo sapiens

SUBMITTER: Raymond Emile Eid

PROVIDER: E-GEOD-11367 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Interleukin (IL)-1 promotes allogeneic T cell intimal infiltration and IL-17 production in a model of human artery rejection.

Rao Deepak A DA Eid Raymond E RE Qin Lingfeng L Yi Tai T Kirkiles-Smith Nancy C NC Tellides George G Pober Jordan S JS

The Journal of experimental medicine 20081215 13

Interleukin (IL) 1alpha produced by human endothelial cells (ECs), in response to tumor necrosis factor (TNF) or to co-culture with allogeneic T cells in a TNF-dependent manner, can augment the release of cytokines from alloreactive memory T cells in vitro. In a human-mouse chimeric model of artery allograft rejection, ECs lining the transplanted human arteries express IL-1alpha, and blocking IL-1 reduces the extent of human T cell infiltration into the artery intima and selectively inhibits IL- ...[more]

PMID: 19075290

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