Induction of muscle-related gene expression in loose connective tissue fibroblasts in response to static tissue stretch

ABSTRACT: Current knowledge of mechanically-induced regulation of gene expression in fibroblast is mostly based on experiments using prolonged cyclical stretching of either cultured cells or load-bearing connective tissues such as tendons and ligaments. In this study, we have examined the effect of static tissue stretch on fibroblasts within loose connective tissue using in vivo and ex vivo models. In an in vivo trunk side bending model, one side of the trunk of the mouse was stretched while the other side was shortened under anesthesia for 90 minutes. In the ex vivo method, whole subcutaneous tissue explants (including skin, subcutaneous muscle and subcutaneous tissue) from both sides of the trunk were incubated ex vivo either stretched or not stretched (shortened) for 24 hours. Tissue stretch

ORGANISM(S): Mus musculus

SUBMITTER: Jeffrey Bond 

PROVIDER: E-GEOD-11371 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress