Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcriptional Response of Candida albicans Cells Following Phagocytosis by Mouse Macrophages

ABSTRACT: Transcriptional profiling of Candida albicans that had been phagocytosed for 1, 2, or 4 hours by either bone marrow-derived mouse macrophaged (BMDM) or immortalized RAW264.7 cells Keywords: Time course Two-condition experiment, phagocytosed cells vs cells maintained in D-10 media. Time course: 1, 2, and 4 hours. Biological replicates: 4 independent experiments at each cell-time combinations.

ORGANISM(S): Candida albicans

SUBMITTER: Andre Nantel

PROVIDER: E-GEOD-11399 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Analysis of PRA1 and its relationship to Candida albicans- macrophage interactions.

Marcil A A Gadoury C C Ash J J Zhang J J Nantel A A Whiteway M M

Infection and immunity 20080714 9

Phagocytosis of Candida albicans by either primary bone marrow-derived mouse macrophages or RAW 264.7 cells upregulated transcription of PRA1, which encodes a cell wall/membrane-associated antigen previously described as a fibrinogen binding protein. However, a pra1 null mutant was still able to bind fibrinogen, showing that Pra1p is not uniquely required for fibrinogen binding. As well, Pra1 tagged with green fluorescent protein did not colocalize with AlexaFluor 546-labeled human fibrinogen, a ...[more]

PMID: 18625733

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Project description:Transcriptional profiling of CDC53 down-regulated Candida albicans cells compared to control cells Keywords: comparative genomic hybridization, genetic modification Candida albicans is an important opportunistic human fungal pathogen, which can cause mucosal as well as systemic infections in immunocompromised patients. Critical for the virulence of C. albicans is its ability to undergo a morphological transition from yeast to hyphal growth mode. Proper induction of filamentation is dependent on the ubiquitination pathway, which targets proteins for proteasome-mediated protein degradation or activates them for signaling events. In the present study, we evaluated the role of ubiquitination in C. albicans by impairing the function of the major ubiquitin-ligase complex SCF. This was done by depleting its backbone, the cullin Cdc53p (orf19.1674), using a tetracycline down-regulatable promoter system. Cdc53p-depleted cells displayed an invasive phenotype and constitutive filamentation under conditions favouring yeast growth mode, both on solid and in liquid media. In addition, these cells exhibited an early onset of cell death, as judged from propidium iodide staining, suggesting that CDC53 is an essential gene in C. albicans. To identify Cdc53p-dependent pathways in C. albicans, a genome-wide expression analysis was carried out that revealed a total of 425 differentially expressed genes (fold change â¥ 2, p-value â¤ 0.05) with 192 up- and 233 down-regulated genes in the CDC53-repressed mutant as compared to the control strain. GO term analysis identified biological processes significantly affected by Cdc53p depletion, including amino acid starvation response, with 14 genes being targets of the transcriptional regulator Gcn4p, and reductive iron transport. These results indicate that Cdc53p enables C. albicans to adequately respond to environmental signals. Two-strain experiment, CDC53-repressible cells vs. control cells, both grown in the presence of 20 Âµg/ml Doxycycline. Four independent biological replicates of total RNA of each strain, dye-swap.

Dataset Information

Transcriptional Response of Candida albicans Cells Following Phagocytosis by Mouse Macrophages

Publications

Analysis of PRA1 and its relationship to Candida albicans- macrophage interactions.

Similar Datasets

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