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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling by array of human dermal fibroblasts to determine passage dependent gene expression

ABSTRACT: Human umbilical vein endothelial cells (HUVECs) formed capillary structures when co-cultured with normal human dermal fibroblasts (NHDFs). HUVEC competence and NHDF supportiveness of cord formation were found to be highly cell-passage dependent with the early passage cells forming more angiogenic cord structures. We thus profiled gene expression in NHDFs with different passages to understand the molecular mechanisms underlying the in vitro angiogenesis control. Experiment Overall Design: NHDFs cultured to various passages were assayed for their gene expression using the GeneChip technology.

ORGANISM(S): Homo sapiens

SUBMITTER: Tao Wei

PROVIDER: E-GEOD-11418 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Developing and applying a gene functional association network for anti-angiogenic kinase inhibitor activity assessment in an angiogenesis co-culture model.

Chen Yuefeng Y Wei Tao T Yan Lei L Lawrence Frank F Qian Hui-Rong HR Burkholder Timothy P TP Starling James J JJ Yingling Jonathan M JM Shou Jianyong J

BMC genomics 20080602

<h4>Background</h4>Tumor angiogenesis is a highly regulated process involving intercellular communication as well as the interactions of multiple downstream signal transduction pathways. Disrupting one or even a few angiogenesis pathways is often insufficient to achieve sustained therapeutic benefits due to the complexity of angiogenesis. Targeting multiple angiogenic pathways has been increasingly recognized as a viable strategy. However, translation of the polypharmacology of a given compound ...[more]

PMID: 18518970

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Passage dependent gene expression in normal human dermal fibroblasts

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Gene expression in human umbilical cord endothelial cells following premiR-100 overexpression

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Gene expression in human umbilical cord endothelial cells following FoxP1 knockdown

Project description:To search for genes regulated by the transcription factor Forkhead box Protein P1 (FoxP1) in endothelial cells, we transfected human umbilical cord endothelial cells (HUVECs) with a combination of three siRNA oligonucleotides directed against human FoxP1 . Human umbilical vein endothelial cells (HUVECs) were isolated from donated umbilical cords, pooled from two donors and cultivated up to passage 5. For transfection with FoxP1-siRNA cells were cultured to 70% confluence and transfected with a combination of three FoxP1-targeting siRNAs or an irrelevant control oligonucleotide (all from invitrogen) using Lipofectamin RNAiMax (Invitrogen) according to the manufacturers instructions. Total RNA was isolated 48h after transfection.

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