Transcription profiling of human organ culture of hyperparathyroidism reveals that phosphate stimulates parathyroid cell proliferation and pth release

ABSTRACT: The pathogenesis of primary hyperparathyroidism (I-HPT) and secondary hyperparathyroidism (II-PTH) remains to be elucidated. To characterize their pathophysiology, we investigated the effects of calcium and phosphate on cell proliferation and PTH release in an organ culture of parathyroid tissues. Dissected parathyroid tissues obtained from patients with I-HPT (adenoma) or II-PTH (nodular hyperplasia) were precultured on a collagen-coated membrane for 1-4 week. After exchanging the medium for one containing various concentrations of phosphate, PTH release and [3H]thymidine incorporation were studied. In contrast to dispersed parathyroid cells cultured in a monolayer, calcium decreased PTH release in a concentration-dependent manner in parathyroid tissues. Furthermore, when parathyroid tiss

ORGANISM(S): Homo sapiens

SUBMITTER: Kanji Sato 

PROVIDER: E-GEOD-11423 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress