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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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OsGLK1 regulated genes in rice

ABSTRACT: Transcription factors encoded by GLK genes are putative positive regulators of chloroplast development. To identify the potential downstream genes regulated by OsGLK1, we performed the rice 44k oligo microarray analysis. Keywords: over-expression of full-length cDNAs Total RNA was extracted from calluses derived from T1 seeds of the OsGLK1 full length cDNA overexpression line and the control line grown on the N6D medium with 30 mg/l of Hyg for 14 days at 30˚C, and subjected to 44k oligo-DNA microarray with 4 biological replicates.

ORGANISM(S): Oryza sativa

SUBMITTER: Hidemitsu Nakamura

PROVIDER: E-GEOD-11451 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Ectopic overexpression of the transcription factor OsGLK1 induces chloroplast development in non-green rice cells.

Nakamura Hidemitsu H Muramatsu Masayuki M Hakata Makoto M Ueno Osamu O Nagamura Yoshiaki Y Hirochika Hirohiko H Takano Makoto M Ichikawa Hiroaki H

Plant & cell physiology 20091006 11

For systematic and genome-wide analyses of rice gene functions, we took advantage of the full-length cDNA overexpresser (FOX) gene-hunting system and generated >12 000 independent FOX-rice lines from >25 000 rice calli treated with the rice-FOX Agrobacterium library. We found two FOX-rice lines generating green calli on a callus-inducing medium containing 2,4-D, on which wild-type rice calli became ivory yellow. In both lines, OsGLK1 cDNA encoding a GARP transcription factor was ectopically over ...[more]

PMID: 19808806

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