Global gene transcription analysis of Anaplasma phagocytophilum in tick and human cells using tiling microarrays

ABSTRACT: A whole genome tiling microarray based on the published sequence of the Anaplasma phagocytophilum (Ap) strain Hz genome was used to measure transcriptional activity of Ap grown to late stage in three cell lines representing the diversity of host cells naturally infected by Ap: two human cell lines, HL-60 (promyelocytic leukocyte) and HMEC-1 (microvascular endothelial), and the tick (Ixodes scapularis) cell line ISE6. Keywords: cell type comparison Total RNA was isolated from Ap (HZ strain) infected cells, used to make biotinylated cDNA fragments, and hybridized to Ap whole genome tiling arrays to measure relative abundance of mRNA transcripts. .CEL files generated by the University of Minnesota’s microarray facility were joined to Affymetrix BPMAP files specific to the tiling array using

ORGANISM(S): Anaplasma phagocytophilum HZ

SUBMITTER: Michael Herron 

PROVIDER: E-GEOD-11487 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress