Transcription profiling of mouse BALB/c macrophages housing multiplying Leishmania amazonensis amastigotes

ABSTRACT: In mammals, resident dermal macrophages (MΦs) are subverted by Leishmania (L.) amazonensis amastigotes as host cells permissive for parasite multiplication. These Leishmania are living within a communal parasitophorous vacuole (PV) and are expected to trigger unique MΦ transcriptional signatures. We performed a transcription profiling of mouse MΦs harboring amastigotes to get insights into their reprogramming as host cells for parasite multiplication. BALB/c mouse bone marrow-derived MΦs were either loaded or not with four amastigotes on average. Twenty four hours later, when amastigotes multiply, total RNA from MΦ cultures was prepared, amplified and hybridized onto Affymetrix Mouse430_2 GeneChips®. The outcome recorded a total of 1,248 probe-sets showing significant differential ex

ORGANISM(S): Mus musculus

SUBMITTER: Eric Prina 

PROVIDER: E-GEOD-11497 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress