Transcription profiling of mouse isolated air pouch membranes after injection of monosodium urate crystals

ABSTRACT: Objective. To identify novel monosodium urate (MSU) crystal-induced mRNAs by transcript profiling of isolated murine air pouch membranes. Methods. Nine hours after injecting crystals into air pouches, membranes were meticulously dissected away from the adjacent soft tissues. mRNA expression differences between inflamed and control membranes were determined by oligonucleotide microarray analysis. Induction of selected mRNAs was validated by real-time relative quantitative reverse transcriptase PCR (qPCR) in pouch membranes and murine peritoneal macrophages. Results. Eleven of the 12 most highly upregulated mRNAs related to innate immunity and inflammation. They included mRNAs encoding histidine decarboxylase (the enzyme that synthesizes histamine), interleukin (IL)-6, the cell surface recep

ORGANISM(S): Mus musculus

SUBMITTER: Joseph Menetski 

PROVIDER: E-GEOD-11498 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress