Transcription profiling of composite bone marrow from healthy humans to investigate age related gene expression changes

ABSTRACT: Human bone marrow is a complex, diversified and well-organized hematopoietic network changing composition with age. The purpose of this study was to analyze variations in relative precursor B cell abundance in bone marrow with age by means of global gene expression profiling. RNA was isolated from composite bone marrow from 25 healthy children, adolescents and adults age 2 months to 28 years. As reference transcript for precursor B cells we used recombination activating gene RAG1 exploring the data for other transcripts showing the same profile as RAG1 with age. We identified 54 genes with correlated expression profiles to RAG1 (r ? 0.9, p = 0), characterized by high expression at 3 - 20 months followed by a fast decline to lower signal levels maintained until early adulthood. Immunophenot

ORGANISM(S): Homo sapiens

SUBMITTER: Kristin Jensen 

PROVIDER: E-GEOD-11504 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress