Chromatin immunoprecipitation profiling of human breast cancer cell lines and tissues to identify novel estrogen receptor-{alpha} binding sites and estradiol target genes

ABSTRACT: Estrogen receptor-{alpha} (ER{alpha}) and its ligand estradiol play critical roles in breast cancer growth and are important therapeutic targets for this disease. Using chromatin immunoprecipitation (ChIP)-on-chip, ligand-bound ER{alpha} was recently found to function as a master transcriptional regulator via binding to many cis-acting sites genome-wide. Here, we used an alternative technology (ChIP cloning) and identified 94 ER{alpha} target loci in breast cancer cells. The ER{alpha}-binding sites contained both classic estrogen response elements and nonclassic binding sequences, showed specific transcriptional activity in reporter gene assay, and interacted with the key transcriptional regulators, including RNA polymerase II and nuclear receptor coactivator-3. The great majority of the b

ORGANISM(S): Homo sapiens

SUBMITTER: Zhihong Lin 

PROVIDER: E-GEOD-11506 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress