Transcription profiling of human platelets from sickle cell patients reveals changes in arginine metabolic pathways

ABSTRACT: In sickle cell disease, ischemia-reperfusion injury and intravascular hemolysis produce endothelial dysfunction and vasculopathy characterized by reduced nitric oxide (NO) and arginine bioavailability. Recent functional studies of platelets in patients with sickle cell disease reveal a basally activated state, suggesting that pathological platelet activation may contribute to sickle cell disease vasculopathy. Studies were therefore undertaken to examine transcriptional signaling pathways in platelets that may be dysregulated in sickle cell disease. We demonstrate and validate here the feasibility of comparative platelet transcriptome studies on clinical samples from single donors, by the application of RNA amplification followed by microarray-based analysis of 54,000 probe sets. Data minin

ORGANISM(S): Homo sapiens

SUBMITTER: Xiuli Xu 

PROVIDER: E-GEOD-11524 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress