Transcriptional profiling of Rapamycin treatment in yeast cells lacking the Ptc1 protein phosphatase.

ABSTRACT: We are interested in the study of the already reported sensitivity of yeast cells lacking the type 1 protein phosphatase Ptc1 to the drug rapamycin. In order to carry out our studies, we analyzed the changes in the transcription profile that a short-term incubation with rapamycin (200 ng/mL) has in both, wild type and ptc1 cells. It has been shown that inhibition of the TOR pathway by treatment with rapamycin has profound effects on the global transcriptional profile. We confirmed the previously described transcription changes induced by the drug in wild type cells. Under our working conditions rapamycin increased, in wild type cells, at least 2-fold the expression of 667 genes, whereas it decreased the mRNA level of 721 genes (13.8% and 14.9% of genes with measurable expression level, res

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Antonio Casamayor 

PROVIDER: E-GEOD-11530 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress