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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Response of Escherichia coli and glutathion mutants to Cadmium

ABSTRACT: We investigated the impact of cadmium on the global transcriptome of E. coli wild type, â??gshA and â??gshB mutant cells to evaluate the molecular basis of cadmium toxicity in the presence or absence of cellular thiols. This global transcriptome analysis were done with cells synthezising GSH (wild type), gamma-glutamyl-cysteine (â??gshB mutant) or neither of the two cellular thiols (â??gshA mutant) under the influence of 100 ÂµM Cd(II). E. coli cells, wild type, â??gshA and â??gshB mutant strain, were grown at 37 Â°C . At a cell turbidity of 100 Klett, cells were treated for 10 min with 100 ÂµM Cd(II) or no metal as a control in TMM medium. Total RNA was extracted, DNAse digested and reverse-transcribed with either Cy3- or Cy5-labeled dCTP. Both labeled cDNA were hybridized to a slide at 42 Â°C. All experiments were performed with three independent biological repeats.

ORGANISM(S): Escherichia coli

SUBMITTER: Cornelia GroÃ?e

PROVIDER: E-GEOD-11562 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Cadmium toxicity in glutathione mutants of Escherichia coli.

Helbig Kerstin K Grosse Cornelia C Nies Dietrich H DH

Journal of bacteriology 20080606 15

The higher affinity of Cd(2+) for sulfur compounds than for nitrogen and oxygen led to the theoretical consideration that cadmium toxicity should result mainly from the binding of Cd(2+) to sulfide, thiol groups, and sulfur-rich complex compounds rather than from Cd(2+) replacement of transition-metal cations from nitrogen- or oxygen-rich biological compounds. This hypothesis was tested by using Escherichia coli for a global transcriptome analysis of cells synthesizing glutathione (GSH; wild typ ...[more]

PMID: 18539742

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