Transcription profiling of mouse B cells transfected with ELL2 vs. controls reveals transcription elongation factor ELL2 influences splicing versus poly(A) site choice in the Ig heavy chain gene

ABSTRACT: Processing of Immunoglobulin heavy chain (IgH) mRNA is a paradigm for competition between splicing and polyadenylation. In plasma cells pre-mRNA is polyadenylated mainly at the promoter-proximal secretory site while B-cells utilize a cryptic 5â?? splice site in the last secretory-specific exon; these are mutually exclusive events for all IgH pre-mRNAs. Transcription elongation factor ELL2, more abundant in plasma cells relative to B-cells, was down-modulated by overexpression of heterogenous ribonucleoprotein F, a condition which reduced production of secretory IgH mRNA. Transfection of B-cells with ELL2 and the IgH reporter showed an accelerated use of the secretory poly(A) site, positioned in competition with the splice to M1; a small interfering RNA to ELL2 reduced expression of IgH

ORGANISM(S): Mus musculus

SUBMITTER: kathleen martincic 

PROVIDER: E-GEOD-11586 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress