Transcription profiling by array of Arabidopsis seedlings mutant for pif1

ABSTRACT: Arabidopsis bHLH transcription factor PIF1 has been shown function as a negative regulator of Chlorophyll biosynthesis. Pif1-2 mutant accumulate more protochlorophylide during prolonged dark growth condations, therefore the comparation between Wt and pif1-2 mutant will help us find the PIF1 target genes in Chlorophyll biosynthesis pathway at genome wide level under the dark grown conditions. We used microarrays to detail the global gene expression under the regulation of PIF1. Experiment Overall Design: 4 days dark grown Wt and pif1-2 seedlings were collected for isolation RNA. Standard Affymetrix protocal and 25K Affymetrix chip (ATH1) were used for Microarry hybridition.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Enamul Huq 

PROVIDER: E-GEOD-11594 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress