Transcription profiling of fertilized mouse eggs reveals Ca2+ oscillatory pattern in fertilized mouse eggs affects gene expression and development to term.

ABSTRACT: The Ca2+ oscillations initiated by the fertilizing sperm (but terminating concomitant with pronucleus formation) apparently ensure that the events constituting egg activation occur in the correct temporal order; early events (e.g., cortical granule exocytosis) require fewer oscillations than later events (e.g., recruitment of maternal mRNA). Whether the Ca2+ signaling events impact long-term development, in particular development to term, is unknown. Using fertilized eggs that have undergone the first few Ca2+ oscillations, we developed procedures that result either in inhibiting or stimulating the natural pattern of Ca2+ signaling of inseminated eggs. Although the incidence of development to the blastocyst stage is unaltered by these procedures, fewer offspring are born following embryo t

ORGANISM(S): Mus musculus

SUBMITTER: Hua Pan 

PROVIDER: E-GEOD-11687 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress