Expression profiling of cotton (Gossypium hirsutum L. TM1) fiber transcriptome during the development

ABSTRACT: In order to study gene expression at the genomic level during elongation and secondary cell wall synthesis of upland cotton fiber, oligonucleotide microarrays were employed. RNA was isolated from fibers in 7 different time points beginning prior to peak fiber expansion, continuing through termination of fiber expansion and ending at peak cellulose synthesis (5, 8, 10, 14, 17, 21, and 24dpa). The arrays contained ~25,000 oligonucleotides representing ~12,200 genes designed from a fiber EST database during peak cell expansion. Dynamic changes in gene expression were analyzed in a developmental context to identify stage-specific biological processes and pathways likely to be crucial to cell polar elongation or cellulose biosynthesis and secondary cell wall biogenesis. Genes with significant c

ORGANISM(S): Gossypium hirsutum

SUBMITTER: Brad Sickler 

PROVIDER: E-GEOD-11693 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress