Induction of adaptive changes in the Streptococcus agalactiae transcriptome by human blood

ABSTRACT: In the present investigation, we conducted a bacterial transcriptome dynamics analysis during GBS incubation with human blood. We observed a large modification in the expression of genes involved in transcriptional regulation and in the metabolism of carbohydrates, purins/pyrimidins and inorganic ions, and an up-regulation of genes involved in attachment and plasminogen contact. We also observed differences in the expression of GBS genes in blood at 40° relative to 37°C. The serotype III GBS reference strain NEM316 was cultivated in Todd Hewitt broth supplemented with 0.2% yeast extract (THY) in 5% CO2 at 37°C until OD reached 0.7. Culture was harvested by centrifugation 8 minutes at 4000 x g at 37°C, then suspended in phosphate-buffered saline (PBS). Fresh heparinized human blood was obta

ORGANISM(S): Streptococcus agalactiae

SUBMITTER: Izabela Sitkiewicz 

PROVIDER: E-GEOD-11705 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress