Suppressing the barley uroporphyrinogen gene by Ds activation tagging element generates developmental photosensitivity

ABSTRACT: Chlorophyll production involves the synthesis of photoreactive intermediates that are toxic when in excess due to the production of reactive oxygen species (ROS). A novel activation tagged barley mutant is described that results from antisense suppression of a uroporphyrinogen III synthase (Uros) gene, the product of which catalyses the sixth step in the synthesis of chlorophyll and heme. In homozygous mutant plants uroporphyrin(ogen) I accumulates by spontaneous cyclization of hydroxyl methylbilane, the substrate of Uros. Accumulation of this tetrapyrrole intermediate results in photosensitive cell death due to the production of reactive oxygen species. The efficiency of Uros gene suppression is developmentally regulated, being most effective in mature seedling leaves compared with newly

ORGANISM(S): Hordeum vulgare

SUBMITTER: Andrew Spriggs 

PROVIDER: E-GEOD-11776 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress