Biologic Phenotyping of the Human Small Airway Epithelial Response to Cigarette Smoking

ABSTRACT: The first changes associated with smoking are in the small airway epithelium (SAE). Given that smoking alters SAE gene expression, but only a fraction of smokers develop chronic obstructive pulmonary disease (COPD), we hypothesized that assessment of SAE genome-wide gene expression would permit biologic phenotyping of the smoking response, and that a subset of healthy smokers would have a “COPD-like” SAE transcriptome. SAE (10th-12th generation) was obtained via bronchoscopy of healthy nonsmokers, healthy smokers and COPD smokers and microarray analysis was used to identify differentially expressed genes. Individual responsiveness to smoking was quantified with an index representing the % of smoking-responsive genes abnormally expressed (ISAE), with healthy smokers grouped into “high” and

ORGANISM(S): Homo sapiens

SUBMITTER: Yael Strulovici-Barel 

PROVIDER: E-GEOD-11784 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress