Transcription profiling ofrat cortical cells transfected with Math2 reveals Prg1 is regulated by the basic helix-loop-helix transcription factor Math2

ABSTRACT: Math2 (NEX-1/NeuroD6) is a member of the bHLH transcription factor family and is involved in neuronal differentiation and maturation. In the present study, we identified the genes targeted by Math2 using DNA microarrays and cultured rat cortical cells transfected with Math2. Of the genes regulated by Math2, we focused on plasticity-related gene 1 (Prg1). Prg1 expression induced by Math2 was confirmed in cultured rat cortical cells and PC12 cells analyzed by real-time quantitative PCR. Examining the promoter region of rat Prg1, we found four E-boxes designated -E1 to -E4 (CANNTG) which were recognized by the bHLH transcription factor. Using chromatin immunoprecipitation (ChIP) assays, we found that Math2 directly bound to the E-box(es) in the Prg1 promoter. The reporter assay of Prg1 showed

ORGANISM(S): Rattus norvegicus

SUBMITTER: Kou Takahashi 

PROVIDER: E-GEOD-11829 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress