Transcription profiling by array of human female interstitial cystitis urothelial cells after inducing differentiation

ABSTRACT: Purpose: Evaluate gene expression profiles after inducing differentiation in cultured interstitial cystitis (IC) and control urothelial cells. Materials and Methods: Bladder biopsies were taken from IC patients and controls (women having surgery for stress incontinence). Primary cultures were grown in Keratinocyte Growth Medium with supplements. To induce differentiation, in some plates the medium was changed to DMEM-F12 with supplements. RNA was analyzed with Affymetrix chips. Three nonulcer IC patients were compared with three controls. Results: After inducing differentiation, 302 genes with a described function were altered at least 3-fold with p <0.01 in both IC and control cells. Functions of the162 upregulated genes included cell adhesion (e.g. claudins, occludin, cingulin);

ORGANISM(S): Homo sapiens

SUBMITTER: Deborah Erickson 

PROVIDER: E-GEOD-11839 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress