ShRNA profiling of human Colo16 cells knocked down for Downstreams of hnRNP A2

ABSTRACT: Limited knowledge of the downstream targets of hnRNP A2/B1 has, however, precluded a clear understanding of their roles in cancer cell growth. To define the pathways in which this protein acts we have now carried out microarray experiments with total RNA from Colo16 epithelial cells transfected with an shRNA that markedly suppresses hnRNP A2/B1 expression. The microarray data identified 123 genes, among 22283 human gene probe sets, with altered expression levels in hnRNP A2/B1-depleted cells. Ontological analysis showed that many of these downstream targets are involved in regulation of the cell cycle and cell proliferation and that this group of proteins is significantly over-represented amongst the affected proteins. The changes detected in the microarray experiments were confirmed by re

ORGANISM(S): Homo sapiens

SUBMITTER: Yaowu He 

PROVIDER: E-GEOD-11865 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress