Transcription profiling of Saccharomyces cerevisiae to investigate the role of rad23/rad4 protein complex in transcription and DNA repair in yeast

ABSTRACT: The Rad23/Rad4 protein complex plays a major role in DNA damage recognition during nucleotide excision repair (NER) in yeast. We recently showed that two distinct pathways contribute to efficient NER in yeast. The first operates independently of de novo protein synthesis and requires a nonproteolytic function of the 19S regulatory complex of the 26S proteasome and Rad23. The second pathway requires de novo protein synthesis, and relies on the activity of a newly identified E3 ubiquitin ligase that ubiquitinates Rad4 in response to UV. Surprisingly, we found that cells deleted of either Rad23 or Rad4 caused reduced Rad4 and Rad23 mRNA levels respectively. We considered the possibility of an unexpected role of Rad23 and Rad4 in regulating the expression of genes involved in the transcription

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Julia Smirnova 

PROVIDER: E-GEOD-11871 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress