Coordinated posttranscriptional mRNA population dynamics during T-cell activation

ABSTRACT: Although RNA-binding proteins (RBPs) coordinate many key decisions during cell growth and differentiation, the dynamics of RNA–RBP interactions have not been extensively studied on a global basis. We immunoprecipitated endogenous ribonucleoprotein complexes containing HuR and PABP throughout a T-cell activation time course and identified the associated mRNA populations using microarrays. We used Gaussian mixture modeling as a discriminative model, treating RBP association as a discrete variable (target or not target), and as a generative model, treating RBP-association as a continuous variable (probability of association). We report that HuR interacts with different populations of mRNAs during T-cell activation. These populations encode functionally related proteins that are members of the

ORGANISM(S): Homo sapiens

SUBMITTER: Neelanjan Mukherjee 

PROVIDER: E-GEOD-11989 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress