Decapped mRNAs during Arabidopsis Early Flower Development

ABSTRACT: The composition of the transcriptome is regulated by both mRNA synthesis and degradation. One route for mRNA decay is through 5’ decapping, which can be initiated by decapping enzymes and small RNAs. Although decapped RNAs are an important intermediate for mRNA decay, their identity and abundance have never been studied on a large scale. Here we present an experimental method for transcriptome-wide profiling of decapped mRNAs. We applied the method to study the prevalence of decapped transcripts during the early stages of Arabidopsis thaliana flower development. Decapped transcripts were identified for the majority of expressed genes, although at different levels. By comparing decapped RNA levels with steady-state overall transcript levels, our study provides evidence for widespread

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Yuling Jiao 

PROVIDER: E-GEOD-12043 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress