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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptome profiling of Pacific oyster Crassostrea gigas families that differ in tolerance of heat shock

ABSTRACT: ESTs in which transcription levels in oyster gill differed among sampling times after heat shock and between families with high or low survival of heat shock were identified through expression profiling of 1,675 spot pairs Keywords: comparison of families characterized by low or high survival after heat shock Oyster families were characterized by survival after heat shock, exposed to a sub-lethal heat shock, and gill was collected before and at 1, 3, 6, and 24 h afterwards. Differences among sampling times and between family types were assessed using a 2-way analysis of variance. In total, 1,675 ESTs were included in the analysis.

ORGANISM(S): Crassostrea gigas

SUBMITTER: Marine Genomics

PROVIDER: E-GEOD-12070 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptome profiling of Pacific oyster Crassostrea gigas families that differ in tolerance of heat shock

Project description:ESTs in which transcription levels in oyster gill differed among sampling times after heat shock and between families with high or low survival of heat shock were identified through expression profiling of 1,675 spot pairs Keywords: comparison of families characterized by low or high survival after heat shock

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Identification of HSF1- target genes involved in thermal stress in the Pacific oyster Crassostrea gigas by ChIP-seq

Project description:The Pacific oyster Crassostrea gigas, a commercially important species inhabiting the intertidal zone, can tolerate temperature fluctuations. Heat shock transcription factor 1 (HSF1) plays an important role in the process of resistance of thermal stress. However, HSF1 has not been fully characterized in the Pacific oyster. C. gigas with an expansion of heat shock protein (HSP) 70. In this study, we analyzed genes regulated by HSF1 in response to heat shock by Chromatin immunoprecipitation followed sequencing (ChIP-seq), determined the expression patterns of target genes by qRT-PCR, and validated the regulatory relationship between one HSP70 and HSF1. We found 916 peaks corresponding to specific binding sites of HSF1, and peaks were annotated to nearest genes. In Gene Ontology analysis, HSF1 target genes was related to signal transduction, energy production, and response to biotic stimulus. Four HSP70 genes, two HSP40 genes, and one small HSP gene exhibited binding to HSF1. One HSP70 with a binding site in the promoter region was validated to be regulated by HSF1 under heat shock. These results provide a basis for future studies aimed at determining the mechanisms underlying thermal tolerance and provide insights into gene regulation in the Pacific oyster.

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Project description:Transcriptome profiling of Pacific oyster Crassostrea gigas families that differ in tolerance of heat shock

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Systemic Comparison of Heat Shock Response Induced by Heat Shock and a Proteasome Inhibitor in Mouse Fibrosarcoma Cells and Their Thermotolerant Counterparts

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