Transcription profiling of Zebrafish single cell dissected and FACS sorted embryos to investigate gastrointestinal tract development implicates novel genes and pathways

ABSTRACT: Zebrafish (Danio rerio) gutGFP transgenic embryos [Tg(XlEef1a1:GFP)s854] were collected at 4 time points: 2 days post fertilization (dpf), 3, dpf, 4 dpf, 6 dpf. Embryos were dissociated into single cells and sorted by FACS based on GFP expression. RNA was extracted from the different cell populations (Stratagene), amplified (NuGEN Ovation), and hybridized to Affymetrix Zebrafish GeneChips. Experiment Overall Design: Series of 24 samples: 4 time points, 3 replicates, 2 samples per replicate (GFP+ and GFP-).

ORGANISM(S): Danio rerio

SUBMITTER: Carsten Stuckenholz 

PROVIDER: E-GEOD-12189 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress