Differential Streptococcus agalactiae gene expression in human blood according to the blood donors

ABSTRACT: In the present investigation, we conducted a bacterial transcriptome dynamics analysis during GBS incubation with human blood. We observed large modification in the expression of numerous genes, but GBS transcriptome was different with the blood of one donor. More genes were up-regulated and less down-regulated in respect to the other donors. The serotype III GBS reference strain NEM316 was cultivated in Todd Hewitt broth supplemented with 0.2% yeast extract (THY) in 5% CO2 at 37°C until OD reached 0.7. Culture was harvested by centrifugation 8 minutes at 4000 x g at 37°C, then suspended in phosphate-buffered saline (PBS). Fresh heparinized human blood was obtained from one healthy volunteer in accordance with a protocol approved by the Institutional Review Board of the Methodist Hospital

ORGANISM(S): Streptococcus agalactiae

SUBMITTER: Izabela Sitkiewicz 

PROVIDER: E-GEOD-12201 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress