Characterization of an Entamoeba histolytica High-Mobility-Group Box Protein Induced during Intestinal Infection

ABSTRACT: The unicellular eukaryote Entamoeba histolytica is a human parasite that causes amebic dysentery and liver abscess. A genome-wide analysis of gene expression modulated by intestinal colonization and invasion identified an up-regulated transcript that encoded a putative high-mobility-group box (HMGB) protein, EhHMGB1. We tested if EhHMGB1 encoded a functional HMGB protein, and determined its role in control of parasite gene expression. Recombinant EhHMGB1 was able to bend DNA in vitro, a characteristic of HMGB proteins. Core conserved residues required for DNA bending activity in other HMGB proteins were demonstrated by mutational analysis to be essential for EhHMGB1 activity. EhHMGB1 was also able to enhance the binding of human p53 to its cognate DNA sequence in vitro, which is expected f

ORGANISM(S): Entamoeba histolytica

SUBMITTER: Carol Gilchrist 

PROVIDER: E-GEOD-12204 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress