Transcription profiling of S. cerevisiae mRNAs following oxidative stress and DNA damage

ABSTRACT: We subjected yeast to two stresses, oxidative stress, which under current settings induces a fast and transient response in mRNA abundance, and DNA damage, which triggers a slow enduring response. Using microarrays, we performed a transcriptional arrest experiment to measure genome-wide mRNA decay profiles under each condition. Genome-wide decay kinetics in each condition were compared to decay experiments that were performed in a reference condition (only transcription inhibition without an additional stress) to quantify changes in mRNA stability in each condition. We found condition-specific changes in mRNA decay rates and coordination between mRNA production and degradation. In the transient response, most induced genes were surprisingly destabilized, while repressed genes were somewhat

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Ophir Shalem 

PROVIDER: E-GEOD-12221 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress