Integrating chromosomal aberrations and gene expression profiles to dissect rectal cancer

ABSTRACT: Accurate staging of rectal tumors is essential for making the correct treatment choice. In a previous study, we found that loss of 17p, 18q and gain of 8q, 13q and 20q could distinguish adenoma from carcinoma tissue and that loss of 1q was related to lymph node metastasis. In order to find markers for tumor staging, we searched for candidate genes on these specific chromosomes. We performed gene expression microarray analysis on 79 rectal tumors and integrated these data with genomic data from the same sample series (Series GSE7946, Samples GSM194994-GSM195028). We performed supervised analysis to find candidate genes on affected chromosomes and validated the results with qRT-PCR and immunohistochemistry. Approximately 8% of the genes were significantly different between adenomas and carci

ORGANISM(S): Homo sapiens

SUBMITTER: Hans Morreau 

PROVIDER: E-GEOD-12225 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress