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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse mammary gland development

ABSTRACT: The mammary gland develops mainly postnatally, when during pregnancy the epithelium grows out into the mammary fat pad and forms a network of epithelial ducts. During pregnancy, these ducts branch and bud to form alveoli. These alveoli produce the milk during lactation. After 7 days of lactation, involution was induced by force weaning the pups. The newly formed epithelium undergoes apoptosis and is removed from the tissue by neighbouring epithelial cells. Tissue remodelling leads to a morphology resembling a gland of a pre-pregnant mouse. Microarray analysis was used to measure mRNA expression of genes during puberty, pregnancy, lactation and involution in a Balb/c mouse strain. Experiment Overall Design: Total RNA was extracted from the 4th (inguinal) gland after removal of the lymph node. Individual samples represent RNA from one gland of one mouse. Samples were taken in triplicate (i.e. three mice per triplicate) for 18 time points of development.

ORGANISM(S): Mus musculus

SUBMITTER: Torsten Stein

PROVIDER: E-GEOD-12247 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Mouse Mammary Gland Development

Project description:The mammary gland develops mainly postnatally, when during pregnancy the epithelium grows out into the mammary fat pad and forms a network of epithelial ducts. During pregnancy, these ducts branch and bud to form alveoli. These alveoli produce the milk during lactation. After 7 days of lactation, involution was induced by force weaning the pups. The newly formed epithelium undergoes apoptosis and is removed from the tissue by neighbouring epithelial cells. Tissue remodelling leads to a morphology resembling a gland of a pre-pregnant mouse. Microarray analysis was used to measure mRNA expression of genes during puberty, pregnancy, lactation and involution in a Balb/c mouse strain. Keywords: developmental time course

2008-08-01 | GSE12247 | GEO

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Distinct lactation-associated macrophages exist in murine mammary tissue and human milk [RNA-seq: human]

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Distinct lactation-associated macrophages exist in murine mammary tissue and human milk [RNA-seq: mouse]

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Genome-wide transcriptional effects of Pregnancy-Induced Non-coding RNA (PINC)

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2012-08-08 | E-GEOD-38052 | biostudies-arrayexpress

Genome-wide transcriptional effects of Pregnancy-Induced Non-coding RNA (PINC)

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Mammary gland development: cross-species analysis of the mammary gland transcriptome in pregnant or lactating wild type female Sprague Dawley rats.

Project description:Cross-species hybridization analysis of mammary glands during pregnancy and lactation. Results provide insight into putative conserved molecular mechanisms regulating mammary gland development. This study was performed to identify orthologous transcripts that are differentially co-expressed in the mammary gland at 2 stages of development (pregnancy and lactation) in wild type Sprague-Dawley rats. Key points are examined in a time series of Sprague Dawley rat mammary gland development, secretory activation and lactation. Triplicate rat (three biological replicates) at each time point were used for statistical power totalling 12 individual arrays in this study. Rats were as staged pregnant day 1 the day that post coital plug was observed, and similarly, lactation day 1 was the first day after birth. Whole mammary glands No. 4 (inguinal) were obtained from female rats at stages of development: virgin (adulthood, 14 wks of age), Pregnant (5 and 14 days of pregnancy) and Lactating (day 1 and 12 postpartum). The two-color (Cy5/Cy3) microarray experiment was designed to hybridize samples from each group against a common reference, a pool of RNA from mammary gland of three parous or virgin female rats.

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