Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

10 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

AURATUS goldfish environmental genomics projects: lumiestrone in the liver

ABSTRACT: Sexually inactive male goldfish were injected i.p. with lumiestrone (0.5 µg/g) or control vehicle (in charcoal-stripped peanut oil; 5 µL/g) to investigate the biological activity of lumiestrone. A common reference design was used: 4 independent groups of sexually inactive male goldfish treated with 0.5 ug/g lumiestrone hybridized against a common vehicle control. Slides 2, 15, 20, 43.

ORGANISM(S): Carassius auratus

SUBMITTER: Jason Popesku

PROVIDER: E-GEOD-12252 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Lumiestrone is Photochemically Derived from Estrone and may be Released to the Environment without Detection.

Trudeau Vance L VL Heyne Belinda B Blais Jules M JM Temussi Fabio F Atkinson Susanna K SK Pakdel Farzad F Popesku Jason T JT Marlatt Vicki L VL Scaiano Juan C JC Previtera Lucio L Lean David R S DR

Frontiers in endocrinology 20111202

Endocrine disrupting chemicals are adversely affecting the reproductive health and metabolic status of aquatic vertebrates. Estrone is often the dominant natural estrogen in urban sewage, yet little is known about its environmental fate and biological effects. Increased use of UV-B radiation for effluent treatments, and exposure of effluents to sunlight in holding ponds led us to examine the effects of environmentally relevant levels of UV-B radiation on the photodegradation potential of estrone ...[more]

PMID: 22654829

Similar Datasets

AURATUS goldfish environmental genomics projects: quinpirole in the telencephalon

Project description:Sexually mature female goldfish were administered 2 i.p. injections: 1) 0.1% DMSO in 0.6% saline, and; 2) 0.6% saline OR quinpirole (2 ug/g) dissolved in 0.6% saline. A common reference design was used: 4 independent groups of telencephali from sexually mature female goldfish treated with 2 ug/g quinpirole hybridized against a common vehicle control. Slides 8, 16, 24, 40.

2013-03-20 | E-GEOD-14609 | biostudies-arrayexpress

AURATUS goldfish environmental genomics projects: SKF 38393 in the hypothalamus

Project description:Sexually mature female goldfish were administered 2 i.p. injections: 1) 0.1% DMSO in 0.6% saline or SKF 38393 (40 µg/g) dissolved in 0.1% DMSO in 0.6% saline, and; 2) 0.6% saline. A common reference design was used: 4 independent groups of hypothalami from sexually mature female goldfish treated with 40 ug/g SKF 38393 hybridized against a common vehicle control. Slides 9, 19, 33, 44.

2011-08-21 | E-GEOD-14607 | biostudies-arrayexpress

AURATUS goldfish environmental genomics projects: SKF 38393 in the telencephalon

Project description:Sexually mature female goldfish were administered 2 i.p. injections: 1) 0.1% DMSO in 0.6% saline or SKF 38393 (40 µg/g) dissolved in 0.1% DMSO in 0.6% saline, and; 2) 0.6% saline. A common reference design was used: 4 independent groups of telencephali from sexually mature female goldfish treated with 40 ug/g SKF 38393 hybridized against a common vehicle control. Slides 7, 13, 30, 47.

2013-03-20 | E-GEOD-14608 | biostudies-arrayexpress

AURATUS goldfish environmental genomics projects: dopamine depletion in hypothalamus and telencephalon

Project description:Sexually mature (mid-May) adult female goldfish received a single injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP; 50 ug/g body weight at 5 uL/g body weight) or 0.6% saline on day 0 and a single injection of alpha-methyl-p-tyrosine (aMPT; 240 ug/g body weight at 5 uL/g body weight) or 0.6% saline on Day 5 with the goal of severely deplete catecholamine (dopamine and norepinephrine) levels. Hypothalamus tissue was extracted on Day 6. Telencephalon tissue was extracted on Day 6. The objective of this study was to examine gene expression changes in the neuroendocrine brain in response to catecholamine depletion. A common reference design was used for both the hypothalamus and telencephalon groups: Hypothalamus: 3 independent groups of hypothalami from sexually mature female goldfish treated with 50 ug/g MPTP + 240 ug/g aMPT hybridized against a common vehicle control. An additional group was used for a dye-swap. Slides 4, 17, 21, 52. Telencephalon: 3 independent groups of telencephali from sexually mature female goldfish treated with 50 ug/g MPTP + 240 ug/g aMPT hybridized against a common vehicle control. An additional group was used for a dye-swap. Slides 28, 34, 43, 48.

2013-03-20 | E-GEOD-16044 | biostudies-arrayexpress

AURATUS goldfish environmental genomics projects: sulpiride in the telencephalon

Project description:Sexually regressing female goldfish were administered 2 i.p. injections: 1) 6 ug/g sulpiride in 0.1% DMSO and 0.6% saline, and; 2) 0.6% saline Tissues were harvested 5h post-injection. A common reference design was used: 4 independent groups of telencephali from sexually regressing female goldfish treated with 6 ug/g sulpiride hybridized against a common vehicle control. Slides 12, 26, 37, 48

2013-03-20 | E-GEOD-15705 | biostudies-arrayexpress

AURATUS goldfish environmental genomics projects: sulpiride in the hypothalamus

Project description:Sexually regressing female goldfish were administered 2 i.p. injections: 1) 6 ug/g sulpiride in 0.1% DMSO and 0.6% saline, and; 2) 0.6% saline Tissues were harvested 5h post-injection. A common reference design was used: 4 independent groups of hypothalami from sexually mature female goldfish treated with 6 ug/g sulpiride hybridized against a common vehicle control. Slides 6, 15, 28, 44.

2013-03-20 | E-GEOD-15706 | biostudies-arrayexpress

AURATUS goldfish environmental genomics projects: SCH 23390 in the hypothalamus

Project description:Sexually regressing female goldfish were administered 2 i.p. injections: 1) 40 ug/g SCH 23390 in 0.1% DMSO and 0.6% saline, and; 2) 0.6% saline Tissues were harvested 5h post-injection. A common reference design was used: 4 independent groups of telencephali from sexually regressing female goldfish treated with 40 ug/g SCH 23390 hybridized against a common vehicle control. Slides 16, 24, 30, 35

2013-03-20 | E-GEOD-15708 | biostudies-arrayexpress

AURATUS goldfish environmental genomics projects: SCH 23390 in the telencephalon

2013-03-20 | E-GEOD-15707 | biostudies-arrayexpress

AURATUS goldfish environmental genomics projects: fadrozole in the telencephalon

Project description:Sexually mature female goldfish were exposed (waterborne) to 50 ug/L fadrozole or untreated water (control) to examine the global gene expression of fadrozole on the telencephalon. A common reference design was used: 5 independent groups of telencephalonic tissue from sexually mature female goldfish exposed to 50 ug/L of waterborne fadrozole were hybridized against a common pool of unexposed fish. Slides 4, 14, 22, 33, 44.

2010-05-16 | E-GEOD-14558 | biostudies-arrayexpress

Secretoneurin: A new hormone? Auratus: The goldfish environmental genomics project

Project description:The objectives of this study were to examine the effect of secretoneurin (SN) on pituitary gene expression. A mixture of goldfish pituitaries from both males and females were dispersed using Typsin type II and DNase II enzymes. The cells were plated in 24-well culture plates at a density of ~2.5 x 105 cells/well. After at least 12-hr plating period, cells were washed with testing medium and pre-incubated for 1 hr to stabilize basal LH secretion. Dispersed pituitary cells were treated with 10 nM gfSN for 12 hours in static incubation after which the cells were carefully washed by 1X PBS. Total RNA was isolated by utilizing RNeasy Micro Kit (QIAGEN GmbH, Hilden, Germany) following the company’s standard protocol. RNA concentrations were determined using the NanoDrop ND-100 spectrophotometer (Thermo Scientific, Wilmington, DE). cDNA synthesis, cDNA purification and microarray slide hybridization followed our validated and established microarray protocol (Martyniuk et al., 2006; Zhang et al., 2009; Popesku et al., 2010). 4 control and 4 treatment samples were hybridized using dual channel micrarray with cy3 and cy5

2011-10-05 | E-GEOD-32629 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data