Transcription profiling of wild-type or FAN knockout mouse embryos to evaluate the role of FAN in TNF-induced gene expression

ABSTRACT: FAN (Factor associated with neutral sphingomyelinase activation) is an adaptor protein that constitutively binds to TNF-R1. Microarray analysis was performed in fibroblasts derived from wild-type or FAN knockout mouse embryos to evaluate the role of FAN in TNF-induced gene expression. We used microarrays to detail the global programme of gene expression underlying cellularisation and identified distinct classes of up-regulated genes during this process. Experiment Overall Design: MEFs were derived from C57BL/6 embryos that were either wild type or FAN-/-. Cells were either grown in DMEM 0%FCS for 24h or DMEM 0% FCS for 8h followed by incubation in DMEM 0% FCS containing 50ng/ml TNF for 16h. These four conditions were each used to generate total RNA (RNeasy MidiKit, Qiagen) which was sent t

ORGANISM(S): Mus musculus

SUBMITTER: bruno Ségui 

PROVIDER: E-GEOD-12275 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress