Comparison between Saccharomyces cerevisiae HS 10, HS 34 and HS 12, HS 37

ABSTRACT: Gene expression of different flocculent (HS 10 and HS 34) and powdery (HS 12 and HS 37) yeast strains compared to each other during exponential and stationary growth phase was analysed. The isolation of RNA was done by disruption of the cells under liquid nitrogen using mortar and pistil and then the Qiagen RNeasy Midi Kit with some modifications within the manufacturers´ protocol and the Qiagen RNase-Free DNase Set were applied. 6 µg of the total RNA per sample was used for each microarray experiments. The indirect labelling by the tyramide-signal-amplification method (MicromaxTM TSATM labelling and detection Kit from Perkin Elmer life sciences) was used to increase the Cy3 and Cy5 signals of microarray detection. Each cDNA containing Biotin- and Fluorescein-nucleotides respectively was

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Franziska Heine 

PROVIDER: E-GEOD-12369 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress