Transcription profiling of human primary fibroblasts and keratinocytes made into human induced pluripotent stem (hiPS) cells - a high efficiency system for the generation and study of human induced pluripotent stem cells

ABSTRACT: Direct reprogramming of human fibroblasts to a pluripotent state has been achieved through ectopic expression of the transcription factors OCT4, SOX2, and either cMYC and KLF4 or NANOG and LIN28. Little is known, however, about the mechanisms by which reprogramming occurs, which is in part limited by the low efficiency of conversion. To this end, we sought to create a doxycycline-inducible lentiviral system to convert primary human fibroblasts and keratinocytes into human induced pluripotent stem (hiPS) cells. hiPS cells generated with this system were molecularly and functionally similar to human embryonic stem (hES) cells, demonstrated by gene expression profiles, DNA methylation status, and differentiation potential. While expression of the viral transgenes was required for several week

ORGANISM(S): Homo sapiens

SUBMITTER: Tim Ahfeldt 

PROVIDER: E-GEOD-12390 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress