Comparative transcriptome analysis between original and evolved recombinant lactose-consuming S. cerevisiae strains

ABSTRACT: The engineering of Saccharomyces cerevisiae strains for lactose utilization has been attempted with the intent of developing high productivity processes for alcoholic fermentation of cheese whey. A recombinant S. cerevisiae flocculent strain that efficiently ferments lactose to ethanol was previously obtained by evolutionary engineering of an original recombinant that displayed poor lactose fermentation performance. In this study, we compared the transcriptomes of the original and the evolved recombinant strains (T1 and T1-E, respectively) growing in lactose, using cDNA microarrays. Microarray data revealed 173 genes whose expression levels differed more than 1.5-fold. About half of these genes were related to RNA mediated transposition. We also found genes involved in DNA repair and recom

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Pedro Guimaraes 

PROVIDER: E-GEOD-12433 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress