Transcription profiling of human cord lining epithelial cells after phage integrase mediated integration

ABSTRACT: The two obstacles that impede a wider application of genetically modified cells expressing therapeutic transgenes for ex vivo gene therapy are the immune mediated rejection of the transplanted cells, combined with their potential to cause iatrogenic oncogenesis. In this study we describe a new cellular vehicle for this form of therapy,; termed the cord lining epithelial cell (CLEC). CLECs are derived from the human amnion and incorporate many of the immunoregulatory functions associated with the fetal/maternal interface. We show that CLECs can be safely transfected by phage φC31 integrase to accomplish site-specific integration of a therapeutic human transgene. We also show that transplanted CLECs are not oncogenic in vivo and can be maintained in immunocompetent mice where acute xeno-rej

ORGANISM(S): Homo sapiens

SUBMITTER: Jaichandran Sivalingam 

PROVIDER: E-GEOD-12510 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress